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GATE Biochemistry Syllabus 2026: IIT Guwahati has released the GATE 2026 Biochemistry syllabus pdf on the official website, gate2026.iitg.ac.in. Candidates can check the official GATE Biochemistry syllabus pdf 2026 on this page. Those appearing for the Biochemistry paper must check the complete GATE 2026 syllabus for their preparation. The GATE 2026 Biochemistry syllabus includes topics such as the organisation of life, enzyme kinetics, biochemical separation techniques, cell structure and organelles, the Immune System and many more. The authority will conduct the Graduate Aptitude Test in Engineering for postgraduate admission and PSU recruitment. The GATE 2026 exam will be held on February 7, 8, 14 and 15, 2026.
Direct link to download the GATE 2026 Biochemistry syllabus
Along with the exam syllabus, candidates can check the GATE 2026 exam pattern. While preparing for the GATE 2026 Biochemistry exam, candidates must understand the exam pattern, the GATE Bio-chemistry syllabus and the marking scheme of various important topics. Read the complete article to know about the GATE syllabus for Biochemistry.
Indian Institute of Technology Guwahati has released the GATE Biochemistry syllabus 2026 on the official website, gate2026.iitg.ac.in. Candidates can check the GATE 2026 Biochemistry syllabus in the table below.
| Topics | Sub-Topics |
| Linear Algebra | Matrices and determinants; Systems of linear equations; Eigen values and Eigen vectors. |
| Calculus | Limits, continuity and differentiability; Partial derivatives, maxima and minima; Sequences and series; Test for convergence |
| Differential Equations | Linear and nonlinear first order ODEs, higher order ODEs with constant coefficients; Cauchy’s and Euler’s equations; Laplace transforms. |
| Probability and Statistics | Mean, median, mode and standard deviation; Random variables; Poisson, normal and binomial distributions; Correlation and regression analysis |
| Numerical Methods | Solution of linear and nonlinear algebraic equations; Integration by trapezoidal and Simpson’s rule; Single step method for differential equations. |
| Chapters | Topics |
| Biochemistry | Biomolecules - structure and function; Biological membranes - structure, membrane channels and pumps, molecular motors, action potential and transport processes; Basic concepts and regulation of metabolism of carbohydrates, lipids, amino acids and nucleic acids; Photosynthesis, respiration and electron transport chain. Enzymes - Classification, catalytic and regulatory strategies; Enzyme kinetics - Michaelis-Menten equation; Enzyme inhibition - competitive, non-competitive and uncompetitive inhibition. |
| Microbiology | Bacterial classification and diversity; Microbial Ecology - microbes in marine, fresh water and terrestrial ecosystems; Microbial interactions; Viruses - structure and classification; Methods in microbiology; Microbial growth and nutrition; Nitrogen fixation; Microbial diseases and host-pathogen interactions; Antibiotics and antimicrobial resistance. |
| Immunology | Innate and adaptive immunity, humoral and cell mediated immunity; Antibody structure and function; Molecular basis of antibody diversity; T cell and B cell development; Antigen-antibody reaction; Complement; Primary and secondary lymphoid organs; Major histocompatibility complex (MHC); Antigen processing and presentation; Polyclonal and monoclonal antibody; Regulation of immune response; Immune tolerance; Hypersensitivity; Autoimmunity; Graft versus host reaction; Immunization and vaccines |
| Chapters | Topics |
| Genetics and Evolutionary Biology | Mendelian inheritance; Gene interaction; Complementation; Linkage, recombination and chromosome mapping; Extra chromosomal inheritance; Microbial genetics - transformation, transduction and conjugation; Horizontal gene transfer and transposable elements; Chromosomal variation; Genetic disorders; Population genetics; Epigenetics; Selection and inheritance; Adaptive and neutral evolution; Genetic drift; Species and speciation. |
| Cell Biology | Prokaryotic and eukaryotic cell structure; Cell cycle and cell growth control; Cellcell communication; Cell signalling and signal transduction; Post-translational modifications; Protein trafficking; Cell death and autophagy; Extra-cellular matrix. |
| Molecular Biology | Molecular structure of genes and chromosomes; Mutations and mutagenesis; Regulation of gene expression; Nucleic acid - replication, transcription, splicing, translation and their regulatory mechanisms; Non-coding and micro RNA; RNA interference; DNA damage and repair. |
| Chapters | Topics |
| Engineering Principles Applied to Biological Systems | Material and energy balances for reactive and non-reactive systems; Recycle, bypass and purge processes; Stoichiometry of growth and product formation; Degree of reduction, electron balance and theoretical oxygen demand |
| Classical Thermodynamics and Bioenergetics | Laws of thermodynamics; Solution thermodynamics; Phase equilibria, reaction equilibria; Ligand binding; Membrane potential; Energetics of metabolic pathways, oxidation and reduction reactions |
| Transport Processes | Newtonian and non-Newtonian fluids, fluid flow - laminar and turbulent; Mixing in bioreactors, mixing time; Molecular diffusion and film theory; Oxygen transfer and uptake in bioreactor, kLa and its measurement; Conductive and convective heat transfer, LMTD, overall heat transfer coefficient; Heat exchangers. |
| Chapters | Topics |
| Bioreaction Engineering | Rate law, zero and first order kinetics; Ideal reactors - batch, mixed flow and plug flow; Enzyme immobilization, diffusion effects - Thiele modulus, effectiveness factor, Damkoehler number; Kinetics of cell growth, substrate utilization and product formation; Structured and unstructured models; Batch, fed-batch and continuous processes; Microbial and enzyme reactors; Optimization and scale up. |
| Upstream and Downstream Processing | Media formulation and optimization; Sterilization of air and media; Filtration - membrane filtration, ultra filtration; Centrifugation - high speed and ultra; Cell disruption; Principles of chromatography - ion exchange, gel filtration, hydrophobic interaction, affinity, GC, HPLC and FPLC; Extraction, adsorption and drying |
| Instrumentation and Process Control | Pressure, temperature and flow measurement devices; Valves; First order and second order systems; Feedback and feed forward control; Types of controllers – proportional, derivative and integral control, tuning of controllers |
| Chapters | Topics |
| Plants | Totipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture and cell suspension culture system - methodology, kinetics of growth and nutrient optimization; Production of secondary metabolites; Hairy root culture; Plant products of industrial importance; Artificial seeds; Somaclonal variation; Protoplast, protoplast fusion - somatic hybrid and cybrid; Transgenic plants - direct and indirect methods of gene transfer techniques; Selection marker and reporter gene; Plastid transformation. |
| Animals | Culture media composition and growth conditions; Animal cell and tissue preservation; Anchorage and non-anchorage dependent cell culture; Kinetics of cell growth; Micro & macrocarrier culture; Hybridoma technology; Stem cell technology; Animal cloning; Transgenic animals; Knock-out and knock-in animals |
| Microbes | Production of biomass and primary/secondary metabolites - Biofuels, bioplastics, industrial enzymes, antibiotics; Large scale production and purification of recombinant proteins and metabolites; Clinical-, food- and industrial- microbiology; Screening strategies for new products. |
| Chapters | Topics |
| Recombinant DNA technology | Restriction and modification enzymes; Vectors - plasmids, bacteriophage and other viral vectors, cosmids, Ti plasmid, bacterial and yeast artificial chromosomes; Expression vectors; cDNA and genomic DNA library; Gene isolation and cloning, strategies for production of recombinant proteins; Transposons and gene targeting. |
| Molecular tools | Polymerase chain reaction; DNA/RNA labelling and sequencing; Southern and northern blotting; In-situ hybridization; DNA fingerprinting, RAPD, RFLP; Site-directed mutagenesis; Gene transfer technologies; CRISPR-Cas; Biosensing and biosensors. |
| Analytical tools | Principles of microscopy - light, electron, fluorescent and confocal; Principles of spectroscopy - UV, visible, CD, IR, fluorescence, FT-IR, MS, NMR; Electrophoresis; Microarrays; Enzymatic assays; Immunoassays - ELISA, RIA, immunohistochemistry; immunoblotting; Flow cytometry; Whole genome and ChIP-sequencing. |
| Computational tools | Bioinformatics resources and search tools; Sequence and structure databases; Sequence analysis - sequence file formats, scoring matrices, alignment, phylogeny; Genomics, proteomics, metabolomics; Gene prediction; Functional annotation; Secondary structure and 3D structure prediction; Knowledge discovery in biochemical databases; Metagenomics; Metabolic engineering and systems biology |
The GATE Exam Pattern 2026 has been released on the official website, gate2026.iitg.ac.in. The exam pattern includes information on the number of questions, types of questions, marking scheme, and the duration of the exam. Candidates can check the GATE 2026 exam pattern in the table below.
Particulars | Details |
Examination Mode | Computer Based Test (Online) |
Duration | 3 Hours |
Number of Papers in GATE 2026 | 30 Papers |
Section |
|
Type of Questions |
|
Design of Questions | The questions are designed to test the listed abilities
|
Number of Questions | 65 Questions (including 10 questions from General Aptitude) |
Total Marks | 100 Marks |
Marking Scheme | All of the questions will be worth 1 or 2 marks |
GATE Negative Marking | Two types of MCQs:
|
Frequently Asked Questions (FAQs)
The syllabus for GATE 2026 Biochemistry includes the Organisation of Life, Enzyme Kinetics, Regulation and Inhibition, Biochemical Separation Techniques, Cell Structure and Organelles, DNA Replication, Transcription and Translation, and the Immune System.
Yes, the GATE 2026 syllabus has been released on the official website, gate2026.iitg.ac.in.
Starting early is the best option for GATE 2026 preparation. With several months available, early preparation allows candidates ample time for thorough revision and mastering of the syllabus.
Candidates can check the detailed GATE 2026 Biochemistry syllabus on this page.
On Question asked by student community
Hello
Just visit the link I am attaching below, so that it will help you to download and practice the papers, so that you can practice well and score well.
Hope it will help you!!!
Hello dear candidate,
You will not receive another rectification mail unless and until there is still an error in your reuploaded signature. if everything is okay your status will change to accepted on the GATE portal.
My advice is that you should keep checking your application status on the official website of GATE 2026.
Thank you.
Hello,
Visit the below website to download the previous 15 years question paper of GATE exam.
https://engineering.careers360.com/articles/last-15-years-gate-papers-solutions
You'll also get the solutions from it. These question papers will help you a lot in your preparation.
All the best.
This is likely a temporary portal or validation issue, not something you did wrong. When candidates try to change category from GEN to OBC-NCL during the GATE correction window, the system checks required fields and sometimes a supporting certificate or fee is needed. If any required data or document is missing, or the server is busy, the portal can show Something went wrong, please try again. Explain to them that first they should log into the GATE portal, open the Correction tab and confirm which fields are editable and whether a payment or document upload is required for category change. Tell them to clear browser cache or try a different browser/device and retry. If the error persists, advise contacting GATE support or the zonal office with a screenshot and timing of the error so officials can check server logs.
Hello,
The GATE 2026 Agricultural Engineering (AG) paper will have a total of 100 marks. Out of this, 15 marks are for General Aptitude and 85 marks are for core Agricultural Engineering subjects.
The marks are generally distributed among major topics like Engineering Mathematics (12–13 marks), Farm Machinery (10–11), Farm Power (14–15), Soil & Water Conservation (12–13), Irrigation & Drainage (10–12), Agricultural Process Engineering (10–12), and Dairy & Food Engineering (9–10 marks).
The official qualifying marks for GATE 2026 are not yet released. However, based on previous years, the cutoff for the Agricultural Engineering paper is expected to be around 25 marks for General, 22.5 for OBC/EWS, and 16.6 for SC/ST/PwD candidates.
Hope it helps !
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