GATE Biochemistry Syllabus 2025 PDF Free Download

GATE Biochemistry Syllabus 2025 PDF Free Download

Edited By Jalla Venkatesh | Updated on Jan 29, 2025 04:06 PM IST | #GATE

GATE Biochemistry Syllabus 2025: IIT Roorkee released the GATE 2025 Biochemistry syllabus pdf on the official website, gate2025.iitr.ac.in. The official GATE Biochemistry syllabus pdf 2025 is provided below. Candidates appearing for the Biochemistry paper must check the complete GATE syllabus 2025 for their preparation. The GATE 2025 Biochemistry syllabus includes topics such as the organisation of life, enzyme kinetics, biochemical separation techniques, cell structure and organelles, the Immune System and many more. The authority will conduct the Graduate Aptitude Test in Engineering for postgraduate admission and PSU recruitment. The GATE 2025 exam will be held on February 1, 2, 15 and 16, 2025.
Direct link to download the GATE 2025 Biochemistry syllabus pdf

GATE Biochemistry Syllabus 2025 PDF Free Download
GATE Biochemistry Syllabus 2025 PDF Free Download

Along with the exam syllabus, candidates can check the GATE 2025 exam pattern. While preparing for the GATE 2025 Biochemistry exam, candidates must understand the exam pattern, the GATE Bio-chemistry syllabus and the marking scheme of various important topics. Read the complete article to know about the GATE syllabus for Biochemistry.

GATE 2025 Biochemistry Syllabus

Indian Institute of Technology Roorkee published the GATE Biochemistry syllabus 2025 on the official website. Candidates can check the GATE 2025 BioTechnology syllabus in the table below.

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GATE Biochemistry Syllabus 2025 for Engineering Mathematics

TopicsSub-Topics
Linear AlgebraMatrices and determinants; Systems of linear equations; Eigen values and Eigen vectors.
CalculusLimits, continuity and differentiability; Partial derivatives, maxima and minima; Sequences and series; Test for convergence
Differential EquationsLinear and nonlinear first order ODEs, higher order ODEs with constant coefficients; Cauchy’s and Euler’s equations; Laplace transforms.
Probability and StatisticsMean, median, mode and standard deviation; Random variables; Poisson, normal and binomial distributions; Correlation and regression analysis
Numerical MethodsSolution of linear and nonlinear algebraic equations; Integration by trapezoidal and Simpson’s rule; Single step method for differential equations.
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GATE Biochemistry Syllabus 2025 for General Biology

ChaptersTopics
BiochemistryBiomolecules - structure and function; Biological membranes - structure, membrane channels and pumps, molecular motors, action potential and transport processes; Basic concepts and regulation of metabolism of carbohydrates, lipids, amino acids and nucleic acids; Photosynthesis, respiration and electron transport chain. Enzymes - Classification, catalytic and regulatory strategies; Enzyme kinetics - Michaelis-Menten equation; Enzyme inhibition - competitive, non-competitive and uncompetitive inhibition.
MicrobiologyBacterial classification and diversity; Microbial Ecology - microbes in marine, fresh water and terrestrial ecosystems; Microbial interactions; Viruses - structure and classification; Methods in microbiology; Microbial growth and nutrition; Nitrogen fixation; Microbial diseases and host-pathogen interactions; Antibiotics and antimicrobial resistance.
ImmunologyInnate and adaptive immunity, humoral and cell mediated immunity; Antibody structure and function; Molecular basis of antibody diversity; T cell and B cell development; Antigen-antibody reaction; Complement; Primary and secondary lymphoid organs; Major histocompatibility complex (MHC); Antigen processing and presentation; Polyclonal and monoclonal antibody; Regulation of immune response; Immune tolerance; Hypersensitivity; Autoimmunity; Graft versus host reaction; Immunization and vaccines
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GATE Biochemistry Syllabus 2025 for Genetics, Cellular and Molecular Biology

ChaptersTopics
Genetics and Evolutionary BiologyMendelian inheritance; Gene interaction; Complementation; Linkage, recombination and chromosome mapping; Extra chromosomal inheritance; Microbial genetics - transformation, transduction and conjugation; Horizontal gene transfer and transposable elements; Chromosomal variation; Genetic disorders; Population genetics; Epigenetics; Selection and inheritance; Adaptive and neutral evolution; Genetic drift; Species and speciation.
Cell BiologyProkaryotic and eukaryotic cell structure; Cell cycle and cell growth control; Cellcell communication; Cell signalling and signal transduction; Post-translational modifications; Protein trafficking; Cell death and autophagy; Extra-cellular matrix.
Molecular BiologyMolecular structure of genes and chromosomes; Mutations and mutagenesis; Regulation of gene expression; Nucleic acid - replication, transcription, splicing, translation and their regulatory mechanisms; Non-coding and micro RNA; RNA interference; DNA damage and repair.

GATE Biochemistry Syllabus 2025 for Fundamentals of Biological Engineering

ChaptersTopics
Engineering Principles Applied to Biological SystemsMaterial and energy balances for reactive and non-reactive systems; Recycle, bypass and purge processes; Stoichiometry of growth and product formation; Degree of reduction, electron balance and theoretical oxygen demand
Classical Thermodynamics and BioenergeticsLaws of thermodynamics; Solution thermodynamics; Phase equilibria, reaction equilibria; Ligand binding; Membrane potential; Energetics of metabolic pathways, oxidation and reduction reactions
Transport ProcessesNewtonian and non-Newtonian fluids, fluid flow - laminar and turbulent; Mixing in bioreactors, mixing time; Molecular diffusion and film theory; Oxygen transfer and uptake in bioreactor, kLa and its measurement; Conductive and convective heat transfer, LMTD, overall heat transfer coefficient; Heat exchangers.

GATE Biochemistry Syllabus 2025 for Bioprocess Engineering and Process Biotechnology

ChaptersTopics
Bioreaction EngineeringRate law, zero and first order kinetics; Ideal reactors - batch, mixed flow and plug flow; Enzyme immobilization, diffusion effects - Thiele modulus, effectiveness factor, Damkoehler number; Kinetics of cell growth, substrate utilization and product formation; Structured and unstructured models; Batch, fed-batch and continuous processes; Microbial and enzyme reactors; Optimization and scale up.
Upstream and Downstream ProcessingMedia formulation and optimization; Sterilization of air and media; Filtration - membrane filtration, ultra filtration; Centrifugation - high speed and ultra; Cell disruption; Principles of chromatography - ion exchange, gel filtration, hydrophobic interaction, affinity, GC, HPLC and FPLC; Extraction, adsorption and drying
Instrumentation and Process ControlPressure, temperature and flow measurement devices; Valves; First order and second order systems; Feedback and feed forward control; Types of controllers – proportional, derivative and integral control, tuning of controllers

GATE Biochemistry Syllabus 2025 for Plant, Animal and Microbial Biotechnology

ChaptersTopics
PlantsTotipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture and cell suspension culture system - methodology, kinetics of growth and nutrient optimization; Production of secondary metabolites; Hairy root culture; Plant products of industrial importance; Artificial seeds; Somaclonal variation; Protoplast, protoplast fusion - somatic hybrid and cybrid; Transgenic plants - direct and indirect methods of gene transfer techniques; Selection marker and reporter gene; Plastid transformation.
AnimalsCulture media composition and growth conditions; Animal cell and tissue preservation; Anchorage and non-anchorage dependent cell culture; Kinetics of cell growth; Micro & macrocarrier culture; Hybridoma technology; Stem cell technology; Animal cloning; Transgenic animals; Knock-out and knock-in animals
MicrobesProduction of biomass and primary/secondary metabolites - Biofuels, bioplastics, industrial enzymes, antibiotics; Large scale production and purification of recombinant proteins and metabolites; Clinical-, food- and industrial- microbiology; Screening strategies for new products.

GATE Biochemistry Syllabus 2025 for Recombinant DNA Technology and Other Tools in Biotechnology

ChaptersTopics
Recombinant DNA technologyRestriction and modification enzymes; Vectors - plasmids, bacteriophage and other viral vectors, cosmids, Ti plasmid, bacterial and yeast artificial chromosomes; Expression vectors; cDNA and genomic DNA library; Gene isolation and cloning, strategies for production of recombinant proteins; Transposons and gene targeting.
Molecular toolsPolymerase chain reaction; DNA/RNA labelling and sequencing; Southern and northern blotting; In-situ hybridization; DNA fingerprinting, RAPD, RFLP; Site-directed mutagenesis; Gene transfer technologies; CRISPR-Cas; Biosensing and biosensors.
Analytical toolsPrinciples of microscopy - light, electron, fluorescent and confocal; Principles of spectroscopy - UV, visible, CD, IR, fluorescence, FT-IR, MS, NMR; Electrophoresis; Microarrays; Enzymatic assays; Immunoassays - ELISA, RIA, immunohistochemistry; immunoblotting; Flow cytometry; Whole genome and ChIP-sequencing.
Computational toolsBioinformatics resources and search tools; Sequence and structure databases; Sequence analysis - sequence file formats, scoring matrices, alignment, phylogeny; Genomics, proteomics, metabolomics; Gene prediction; Functional annotation; Secondary structure and 3D structure prediction; Knowledge discovery in biochemical databases; Metagenomics; Metabolic engineering and systems biology

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GATE 2025 Exam Pattern For Bio-Chemistry

The GATE Exam Pattern 2025 is updated on the official website. Candidates can check the GATE 2025 exam pattern in the table below. The exam pattern includes information on the number of questions, types of questions, marking scheme, and the duration of the exam.

GATE Biochemistry 2025 Exam Pattern

Particulars

Details

Examination Mode

Computer Based Test (Online)

Duration

3 Hours

Number of Papers in GATE 2025

30 Papers

Section

  • General Aptitude (GA)

  • Candidate Selected Subject

Type of Questions

  • Multiple Choice Questions (MCQs)

  • Multiple Select Questions (MSQs)

  • Numerical Answer Type (NAT) Questions

Design of Questions

The questions are designed to test the listed abilities

  • Application

  • Analysis

  • Comprehension

  • Recall

  • Synthesis

Number of Questions

65 Questions (including 10 questions from General Aptitude)

Total Marks

100 Marks

Marking Scheme

All of the questions will be worth 1 or 2 marks

GATE Negative Marking

Two types of MCQs:

  • MCQs - 1 mark for each correct answer; 1/3 mark will be deducted for every wrong answer.

  • MCQs - 2 marks for each correct answer; 2/3 marks will be deducted for every incorrect response. There are no negative marks for Numerical Answer Type (NAT) questions

  • NO negative marking for MSQ & NAT.

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Frequently Asked Questions (FAQs)

1. What is the syllabus for GATE Biochemistry 2025?

The syllabus for GATE 2025 Biochemistry includes the Organisation of Life, Enzyme Kinetics, Regulation and Inhibition, Biochemical Separation Techniques, Cell Structure and Organelles, DNA Replication, Transcription and Translation, and the Immune System.

2. When should I start preparing for GATE 2025?

Starting early is the best option for GATE 2025 preparation. With several months available, early preparation allows candidates ample time for thorough revision and mastering of the syllabus.

3. How can I access the detailed GATE Biochemistry Syllabus 2025?

Candidates can check the detailed GATE 2025 Biochemistry syllabus on this page.

4. Is the GATE Syllabus 2025 Out?

Yes, the authority has published the GATE 2025 Syllabus on the official website.

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Questions related to GATE

Have a question related to GATE ?

Hello Ansh,

Whether you can get admission in the Computer Science (CS) or Electronics and Communication Engineering (ECE) branch depends on several key factors:

  • Entrance Exam Rank/Score : Your performance in entrance exams like JEE Mains, JEE Advanced, state-level exams (like KCET, AP EAMCET, TS EAMCET), or university-specific exams plays a major role. A high rank increases your chances for CS or ECE.

  • Category and Reservation : SC, ST, OBC, EWS, or other reserved categories may have lower cut-off ranks for CS and ECE branches.

  • College Preference : Top colleges like IITs, NITs, IIITs, and top private universities have higher cut-offs for CS and ECE. Mid-tier or private colleges may offer more flexibility with lower ranks.

  • State Quota vs. All India Quota : Home state students often have a better chance under the state quota.

  • Gender-Based Quotas : Some institutions offer female-only seats or relaxed cut-offs for girls.

If you mention your entrance exam name, rank or percentile, category, and state, I can give a more specific answer.


I hope this answer helps you. If you have more queries, feel free to share your questions with us, and we will be happy to assist you.

Thank you, and I wish you all the best in your bright future.

Hello there,

Yes, you can get admission to M.Tech at CBIT without a GATE score by qualifying through TSPGECET.

  • You must have a B.Tech or equivalent degree in a related field.

  • If you have a valid TSPGECET rank, you can apply under the regular counseling process (Category A).

  • If you do not have a TSPGECET rank, you may apply under Category B (management quota), where admission is based on your qualifying degree marks and seat availability.

  • M.Tech specializations at CBIT include Computer Science Engineering, Computer Networks and Information Security, Artificial Intelligence and Data Science, and Information Technology.

  • Cutoff ranks for TSPGECET vary every year depending on competition and seats.

  • Category B admissions do not have a fixed cutoff; selection depends on merit and seat availability.

For exact cutoffs and seat details, you need to check with CBIT or the Telangana State Council of Higher Education during the admission process.


I hope this answer helps you. If you have more queries, feel free to share your questions with us, and we will be happy to assist you.

Thank you, and I wish you all the best in your bright future.

GATE BT (Biotechnology) score of 350, you have a good chance of securing admission to Delhi Technological University (DTU) for M.Tech in Biotechnology or related programs.

DTU M.Tech Admission via GATE

  1. Eligibility Criteria:

    • You must have a valid GATE score in Biotechnology (BT) or a related discipline.

    • A minimum of 60% (or equivalent CGPA) in your B.Tech/B.E (Biotech or related field) from a recognized university.

Cutoff Trends :

DTU's cutoff for GATE BT usually ranges between 300-400 (varies yearly based on competition.

Unfortunately, you've missed the GATE 2025 exam registration deadline, which was initially September 20, 2024, and later extended to October 3, 2024, for regular registration, with a late fee option available until October 7, 2024. Since the exam dates are already scheduled for February 1, 2, 15, and 16, 2025, you won't be able to apply for this year's exam.


However, here are some potential options to consider:


- Prepare for GATE 2026: You can start preparing for the next year's exam, ensuring you meet the eligibility criteria and stay updated on the application process.

- Explore other exams: Look into other entrance exams for postgraduate programs, such as those offered by individual universities or institutions.

- Check eligibility for other programs: If you're interested in pursuing a postgraduate degree, research other programs that don't require GATE scores.


To better prepare for future exams, focus on

- Understanding the syllabus: Familiarize yourself with the exam pattern, syllabus, and marking scheme.

- Creating a study plan: Develop a structured study schedule, covering all relevant topics.

- Practicing with mock tests: Regularly take practice tests to assess your knowledge and identify areas for improvement.


Keep in mind that GATE scores are valid for three years, so if you plan to take the exam in 2026, your score will be valid until 2029.

Hello there,

With a GATE Electrical Engineering (EE) score of 630, getting admission to the M.Tech program in Instrumentation and Integrated Electronics at IIT Kharagpur is unlikely.

Key points:

  • The closing GATE score for this program in 2024 was around 608 for the general category.

  • For reserved categories, cutoffs are lower (OBC around 573, SC around 337, ST around 248).

  • Admission depends on GATE score and sometimes an interview.

Recommendations:

  • You can consider applying to other IITs or NITs where your score may meet the cutoff for similar courses.

  • Explore other reputed institutes offering M.Tech in Instrumentation and Electronics.

  • If this field is important for you, consider preparing to improve your GATE score for the next attempt.


I hope this answer helps you. If you have more queries, feel free to share your questions with us, and we will be happy to assist you.

Thank you, and I wish you all the best in your bright future.


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