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    GATE Biochemistry Syllabus 2027 PDF Free Download

    GATE Biochemistry Syllabus 2027 PDF Free Download

    Simran KashyapUpdated on 06 Aug 2026, 06:30 PM IST

    GATE Biochemistry Syllabus 2027: IIT Madras has released the GATE 2027 Biochemistry syllabus online. Candidates can download the GATE Biochemistry syllabus 2027 PDF from the official website, gate2027.iitm.ac.in. The GATE Biochemistry syllabus covers important topics such as Biochemistry, Microbiology, Genetics, Cellular and Molecular Biology, Engineering Principles Applied to Biological Systems, Recombinant DNA Technology, and more. Aspirants can also check the direct link to download the GATE Biochemistry syllabus 2027 here in this article. The GATE 2027 Biochemistry paper is one of the optional papers under the Life Sciences (XL) paper. The GATE exam 2027 will be conducted on February 6, 7, 13, 14, 20, and 21, 2027. Candidates are advised to check the GATE 2027 exam pattern along with syllabus.
    Direct link to download the GATE 2027 Biochemistry syllabus

    Live | Aug 30, 2026 | 12:35 AM IST
    GATE Biochemistry Syllabus 2027 PDF Free Download
    GATE Biochemistry Syllabus 2027

    GATE 2027 Biochemistry Syllabus

    Indian Institute of Technology Madras has published the GATE Biochemistry syllabus 2027 on the official website, gate2027.iitm.ac.in. Candidates can check the GATE 2027 Biochemistry syllabus in the table below.

    Section

    Topics Included

    Structure and Function of Biomolecules

    Organisation of life; Importance of water; Structure and function of biomolecules – carbohydrates, lipids, proteins, and nucleic acids; Protein structure, folding/misfolding, and function; Myoglobin, hemoglobin, lysozyme, ribonuclease A, carboxypeptidase, and chymotrypsin.

    Enzymes and General Principles of Metabolism

    Enzymes – catalysis, specificity, regulation, enzyme kinetics (up to two substrates), enzyme inhibitors; Regulatory enzymes; Vitamins and coenzymes; General principles of metabolism; Hormonal regulation of metabolism; Generation and utilization of ATP.

    Metabolic Pathways and Associated Disorders

    Glycolysis; TCA cycle; Pentose phosphate pathway; Oxidative phosphorylation; Gluconeogenesis; Fatty acid metabolism; Metabolism of nitrogen-containing compounds – nitrogen fixation, amino acids, and nucleotides; Photosynthesis; Photophosphorylation; Calvin cycle; Metabolic disorders related to sugars, amino acids, and lipids.

    Analytical Techniques

    Buffering against pH changes in biological systems; Henderson-Hasselbalch equation; Ion exchange chromatography; Size exclusion chromatography; Affinity chromatography; Thin layer chromatography (TLC); Paper chromatography; Centrifugation; Characterization of biomolecules by electrophoresis; DNA-protein and protein-protein interaction analysis; Lambert-Beer law; UV-visible spectroscopy; Fluorescence spectroscopy; Next-generation DNA sequencing (NGS); PCR; Site-directed mutagenesis; Antibody-antigen interaction; Immunological techniques – immunodiffusion, immune-electrophoresis, RIA, ELISA, flow cytometry; Monoclonal antibodies and their applications.

    Cell Structure and Function

    Cell structure and organelles; Biological membranes; Transport across membranes; Membrane assembly; Protein targeting and degradation; Biosignalling; Receptor-ligand interaction; Two-component system; Hormones and neurotransmitters; Toxins and ion channels.

    Gene Expression and Its Regulation, and DNA Repair

    DNA replication; Transcription; Translation; DNA damage and repair; Nuclease accessibility methods; Biochemical regulation of gene expression.

    GATE 2027 Exam Pattern For Biochemistry

    The GATE 2027 exam pattern has been released on the official website. It provides details about the number of questions, question types, marking scheme, exam duration, and mode of examination. Candidates should review the GATE 2027 exam pattern before starting their preparation to understand the test format. Check the GATE 2027 exam pattern in the table below.

    GATE Biochemistry 2027 Exam Pattern

    ParticularsDetails

    Examination Mode

    Computer Based Test (Online)

    Duration

    3 Hours

    Number of Papers in GATE 2027

    30 Papers

    Section

    • General Aptitude (GA)

    • Candidate Selected Subject

    Type of Questions

    • Multiple Choice Questions (MCQs)

    • Multiple Select Questions (MSQs)

    • Numerical Answer Type (NAT) Questions

    Design of Questions

    The questions are designed to test the listed abilities

    • Application

    • Analysis

    • Comprehension

    • Recall

    • Synthesis

    Number of Questions

    65 Questions (including 10 questions from General Aptitude)

    Total Marks

    100 Marks

    Marking Scheme

    All of the questions will be worth 1 or 2 marks

    GATE Negative Marking

    Two types of MCQs:

    • MCQs - 1 mark for each correct answer; 1/3 mark will be deducted for every wrong answer.

    • MCQs - 2 marks for each correct answer; 2/3 marks will be deducted for every incorrect response. There are no negative marks for Numerical Answer Type (NAT) questions

    • NO negative marking for MSQ & NAT.

    Frequently Asked Questions (FAQs)

    Q: What is the syllabus for GATE Biochemistry 2027?
    A:

    The syllabus for GATE 2027 Biochemistry includes the Organisation of Life, Enzyme Kinetics, Regulation and Inhibition, Biochemical Separation Techniques, Cell Structure and Organelles, DNA Replication, Transcription and Translation, and the Immune System.

    Q: When should I start preparing for GATE 2027?
    A:

    Starting early is the best option for GATE 2027 preparation. With several months available, early preparation allows candidates ample time for thorough revision and mastering of the syllabus.

    Q: How can I access the detailed GATE Biochemistry Syllabus 2027?
    A:

    Candidates can check the detailed GATE 2027 Biochemistry syllabus on this page.

    Q: Is the GATE Syllabus 2027 Out?
    A:

    Yes, the GATE 2027 syllabus has been released on the official website.

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