GATE Life Science Syllabus 2025 - Check Here

GATE Life Sciences Syllabus 2025 - IIT Roorkee has released the GATE 2025 life science syllabus pdf on the official website gate2025.iitr.ac.in. Candidates can check the GATE life sciences syllabus 2025 on this page. Candidates must cover all the topics mentioned in the GATE XL syllabus 2025 to score well in the Graduate Aptitude Test in Engineering. Understanding the GATE 2025 Life Sciences syllabus is the first step in preparing for the GATE exam. IIT Roorkee will conduct the GATE 2025 exam in online mode for 30 papers. With the help of GATE syllabus, candidates can know exactly what to prepare and what to skip.
Direct link to download the GATE 2025 Life Sciences Syllabus

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Along with the GATE 2025 Life Sciences syllabus, candidates can also check the GATE exam pattern released by the authorities. The GATE Life Sciences 2025 syllabus comprises topics from Chemistry, Biochemistry, Botany, Microbiology, Zoology and Food Technology. The authorities will prepare the GATE question papers as per the GATE syllabus 2025 for Life Sciences. Check out the complete article for more details about the GATE Life Sciences syllabus 2025.

GATE 2025 Life Science Syllabus

Candidates preparing for the upcoming GATE entrance exam can check the GATE exam syllabus for life science 2025 on this page. The GATE 2025 life science syllabus pdf consists of the list of topics that aspirants should study during preparation. Candidates are also advised to refer to GATE life science topic-wise weightage to get an idea of high-weightage topics. Moreover, candidates are advised to avoid missing out on any topic mentioned in the GATE syllabus for life science.

GATE Life Sciences Syllabus 2025

The syllabus of GATE Life Sciences comprises various topics as mentioned below.

• XL - U Food Technology

• XL - P Chemistry

• XL - Q Biochemistry

• XL - R Botany

• XL - S Microbiology

• XL - T Zoology

GATE Life Sciences Syllabus 2025 - Chemistry

Section 1: Atomic Structure and Periodicity

Planck’s quantum theory, wave-particle duality, uncertainty principle, comparison between Bohr’s model and quantum mechanical model of hydrogen atom, electronic configuration of atoms and ions. Hund’s rule and Pauli’s exclusion principle.

Periodic table and periodic properties: ionization energy, electron affinity, electronegativity and atomic size.

Section 2: Structure and Bonding

Ionic and covalent bonding, MO and VB approaches for diatomic molecules, VSEPR theory and shape of molecules, hybridization, resonance, dipole moment, structure parameters such as bond length, bond angle and bond energy, hydrogen bonding and van der Waals interactions. Ionic solids, ionic radii and lattice energy (Born-Haber cycle). HSAB principle.

Section 3: s, p and d Block Elements

Oxides, halides and hydrides of alkali, alkaline earth metals, B, Al, Si, N, P, and S. General characteristics of 3d elements. Coordination complexes: valence bond and crystal field theory, color, geometry, magnetic properties and isomerism.

Section 4: Chemical Equilibria

Osmotic pressure, elevation of boiling point and depression of freezing point, ionic equilibria in solution, solubility product, common ion effect, hydrolysis of salts, pH, buffer and their applications. Equilibrium constants (Kc, Kp and Kx) for homogeneous reactions.

Section 5: Electrochemistry

Conductance, Kohlrausch law, cell potentials, EMF, Nernst equation, thermodynamic aspects and their applications.

Section 6: Reaction Kinetics

Rate constant, order of reaction, molecularity, activation energy, zero, first and second-order kinetics, catalysis and elementary enzyme reactions. Reversible and irreversible inhibition of enzymes.

Section 7: Thermodynamics

Qualitative treatment of state and path functions, First law, reversible and irreversible processes, internal energy, enthalpy, Kirchoff equation, heat of reaction, Hess’s law, heat of formation. The second law is entropy and free energy. Gibbs-Helmholtz equation, free energy change and spontaneity, Free energy changes from equilibrium constant.

Section 8: Structure-Reactivity Correlations and Organic Reaction Mechanisms

Acids and bases, electronic and steric effects, Stereochemistry, optical and geometrical isomerism, tautomerism, conformers and concept of aromaticity. Elementary treatment of SN1, SN2, E1, E2 and radical reactions, Hofmann/Saytzeff rules, addition reactions, Markownikoff rule and Kharasch effect. Elementary hydroboration reactions. Grignard’s reagents and their uses. Aromatic electrophilic substitutions, and orientation effects as exemplified by various functional groups. Identification of common functional groups by chemical tests.

Section 9: Chemistry of Biomolecules

Amino acids, proteins, nucleic acids and nucleotides. Peptide sequencing by chemical and enzymatic proteolytic methods. DNA sequencing by chemical and enzymatic methods. Carbohydrates (up to hexoses only). Lipids (triglycerides only). Principles of biomolecule purification- Ion exchange and gel filtration chromatography. Identification of these biomolecules and Beer- Lambert’s law.

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GATE 2025 Life Sciences Syllabus - Biochemistry

Section 1

Organization of life; Importance of water; Structure and function of biomolecules: Amino acids, Carbohydrates, Lipids, Proteins and Nucleic acids; Protein structure, folding/misfolding and function; Myoglobin, Hemoglobin, Lysozyme, Ribonuclease A, Carboxypeptidase and Chymotrypsin.

Section 2

Enzyme kinetics, regulation and inhibition; Vitamins and Coenzymes; Bioenergetics and metabolism; Generation and utilization of ATP; Metabolic pathways and their regulation: glycolysis, TCA cycle, pentose phosphate pathway, oxidative phosphorylation, gluconeogenesis, glycogen and fatty acid metabolism; Metabolism of Nitrogen containing compounds: nitrogen fixation, amino acids and nucleotides. Photosynthesis, Calvin cycle.

Section 3

Biochemical separation techniques: ion exchange, size exclusion and affinity chromatography, centrifugation; Characterization of biomolecules by electrophoresis; DNA- protein and protein–protein interactions; UV-visible and fluorescence spectroscopy; Mass spectrometry.

Section 4

Cell structure and organelles; Biological membranes; Action potential; Transport across membranes; Membrane assembly and Protein targeting; Signal transduction; Receptor-ligand interaction; Hormones and neurotransmitters.

Section 5

DNA replication, transcription and translation; DNA damage and repair; Biochemical regulation of gene expression; Recombinant DNA technology and applications: PCR, site-directed mutagenesis, DNA-microarray; Next generation sequencing; Gene silencing and editing.

Section 6

Immune system: Innate and adaptive; Cell of the immune system; Active and passive immunity;

Complement system; Antibody structure, function and diversity; B cell and T Cell receptors; B cell and T cell activation; Major histocompatibility complex; Immunological techniques: Immunodiffusion, immune-electrophoresis, RIA and ELISA, flow cytometry; monoclonal antibodies and their applications.

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GATE XL Syllabus 2025 - Botany

Section 1: Plant Systematics

Botanical nomenclature, history of plant taxonomy, diversity and classification of plants, APG system of plant classification; phylogenetics and cladistics, molecular taxonomy and DNA barcoding; Centers for plant taxonomy and herbaria in India.

Section 2: Plant Anatomy

Anatomy of root, stem and leaves, floral organs, embryo and young seedlings, Primary and secondary meristems, stellar organization, vascular system and their ontogeny, xylem and phloem structure, secondary growth in plants and wood anatomy, plant cell structure and differences from animal cells.

Section 3: Plant development; cell and tissue morphogenesis

Life cycle of an angiosperm, development of male and female gametophyte; cell fate determination and tissue patterning; spacing mechanisms in trichomes and stomata. Embryogenesis, organization and function of shoot and root apical meristems. Transition to flowering: photoperiodism and vernalization, ABC model of floral organ patterning, pollen germination, double fertilization, seed development; Xylem and phloem cell differentiation, photomorphogenesis; phytochrome, cryptochrome, phototropin. Role of auxin, cytokinin, gibberellins, and brassinosteroids on plant development.

Section 4: Plant physiology and biochemistry

Plant water relations, mechanisms of uptake and transport of water, ions, and solutes from soil to plants, apoplastic and symplastic transport mechanisms. Mechanism of stomatal movements, nitrogen metabolism, photosynthesis; C3, C4 and CAM cycles, photorespiration, respiration: glycolysis, TCA cycle and electron transport chain. Plant responses and mechanisms of abiotic stresses including drought, salinity, freezing and heat stress, metal toxicity; the role of abscisic acid in abiotic stresses. Structure and function of biomolecules (proteins, carbohydrates, lipids, nucleic acid), enzyme kinetics. Structure and biosynthesis of major plant secondary metabolites (alkaloids, terpenes, phenylpropanoids, flavonoids). Biosynthesis, mechanism of action and physiological effects of auxin, cytokinin, gibberellic acids, brassinosteroid, ethylene, strigolactone, abscisic acid, salicylic and jasmonic acid. Senescence and programmed cell death.

Section 5: Genetics and genomics

Cell cycle and cell division. Principles of Mendelian inheritance, linkage, recombination, genetic mapping; extrachromosomal inheritance; Introduction to epigenetics; gene silencing- transgene silencing, post-transcriptional gene silencing, miRNA and siRNA; evolution and organization of eukaryotic genome structure, gene expression, gene mutation and repair, chromosomal aberrations (numerical: euploidy and aneuploidy and structural: deletion, duplication, inversion, translocation), transposons. Model organisms for functional genetics and genomics; Introduction to transcriptomics, proteomics and metabolomics.

Section 6: Plant Breeding, Genetic Modification, Genome Editing

Principles, methods – selection, hybridization, heterosis; male sterility, genetic maps and molecular markers, embryo rescue, haploid and doubled haploids, plant tissue culture: micropropagation, embryo culture and in vitro regeneration, somatic embryogenesis, artificial seed, cryopreservation, somaclonal variation, somatic cell hybridization, marker-assisted selection, gene transfer methods viz. direct and vector-mediated, generation of transgenic plants; Introduction to genome editing: CRISPR/Cas9, Cre-Lox system to generate chimeras; plastid transformation; chemical mutagenesis.

Section 7: Economic and Applied Botany

A general account of economically and medicinally important plants- cereals, pulses, plants yielding fibres, timber, sugar, beverages, oils, rubber, pigments, dyes, gums, drugs and narcotics. Economic importance of algae, fungi, lichen and bacteria. Major Indian cash crops. Effect of industrialization on agricultural botany such as plastic on fibre economy. Genetically modified crops and their regulation eg. Bt cotton, Bt brinjal golden rice etc.

Section 8: Plant Pathology

Nature and classification of plant diseases, diseases of important crops caused by fungi, bacteria, nematodes and viruses, and their control measures (chemical and biological) mechanism(s) of pathogenesis, resistance: basal, systemic, induced systemic resistance, gene for gene concept. Molecular detection of pathogens; plant-microbe interactions: symbionts and mycorrhiza, pathogens and pests. Signalling pathways in plant defence response; salicylic acid (SA) and jasmonic acid (JA) in plant-pathogen and plant-herbivore interaction, necrosis; host-parasitic plant interaction (such as Cuscuta).

Section 9: Ecology and Environment

Ecosystems – types, dynamics, degradation, biogeochemical cycles, ecological succession; food webs and energy flow through ecosystem; vegetation types of the world, Indian vegetation types and biogeographical zones, climate and flora endemism; pollution and global climate change, speciation and extinction, biodiversity and conservation strategies, ecological hotspots, afforestation, habitat restoration; plant interactions with other organisms; epiphytes, parasites and endophytes.

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GATE 2025 Life Sciences Syllabus - Microbiology

Section 1: Historical Perspective

Discovery of microbial world; Landmark discoveries relevant to the field of microbiology; Controversy over spontaneous generation; Role of microorganisms in transformation of organic matter and in the causation of diseases.

Section 2: Methods in Microbiology

Pure culture techniques; Principles of microbial nutrition; Enrichment culture techniques for isolation of microorganisms; antigen and antibody detection methods for microbial diagnosis; Light-, phase contrast-, fluorescence- and electron-microscopy; PCR, real-time PCR for quantitation of microbes; Next generation sequencing technologies in microbiology.

Section 3: Microbial Taxonomy and Diversity

Bacteria, Archaea and their broad classification; Eukaryotic microbes: Yeasts, moulds and protozoa; Viruses and their classification; Molecular approaches to microbial taxonomy and phylogeny.

Section 4: Prokaryotic Cells: Structure and Function

Prokaryotic Cells: cell walls, cell membranes and their biosynthesis, mechanisms of solute transport across membranes, Flagella and Pili, Capsules, Cell inclusions like endospores and gas vesicles; Bacterial locomotion, including positive and negative chemotaxis.

Section 5: Microbial Growth

Definition of growth; Growth curve; Mathematical expression of exponential growth phase; Measurement of growth and growth yields; Synchronous growth; Continuous culture; Effect of environmental factors on growth; Bacterial biofilm and biofouling.

Section 6: Control of Microorganisms

Disinfection and sterilization: principles, methods and assessment of efficacy.

Section 7: Microbial Metabolism

Energetics: redox reactions and electron carriers; Electron transport and oxidative phosphorylation; An overview of metabolism; Glycolysis; Pentose-phosphate pathway; Entner-Doudoroff pathway; Glyoxylate pathway; The citric acid cycle; Fermentation; Aerobic and anaerobic respiration; Chemolithotrophy; Photosynthesis; Calvin cycle; Biosynthetic pathway for fatty acids synthesis; Common regulatory mechanisms in synthesis of amino acids; Regulation of major metabolic pathways.

Section 8: Microbial Diseases and Host-Pathogen Interaction

Normal microbiota; Classification of infectious diseases; Reservoirs of infection; Nosocomial infection; Opportunistic infections; Emerging infectious diseases; Mechanism of microbial pathogenicity; Nonspecific defence of host; Antigens and antibodies; Humoral and cell-mediated immunity; Vaccines; passive immunization; Immune deficiency; Human diseases caused by viruses, bacteria, and pathogenic fungi.

Section 9: Chemotherapy/Antibiotics

General characteristics of antimicrobial drugs; Antibiotics: Classification molecular mechanism of mode of action and resistance; Antifungal and antiviral drugs.

Section 10: Microbial Genetics

Types of mutation; UV and chemical mutagens; Selection of mutants; Ames test for mutagenesis; Bacterial genetic system: transformation, conjugation, transduction, recombination, plasmids, transposons; DNA repair; Regulation of gene expression: repression and induction; Operon model; Bacterial genome with special reference to E.coli; Phage λ and its life cycle; RNA; mutation in virus genomes, virus recombination and reassortment; Basic concept of microbial genomics.

Section 11: Microbial Ecology

Microbial interactions; Carbon, sulphur and nitrogen cycles; Soil microorganisms associated with vascular plants; Bioremediation; Uncultivable microorganisms; the basic concept of metagenomics and metatranscriptomics.

Life Sciences GATE Syllabus 2025 - Zoology

Section 1: Animal Diversity

Distribution, systematics and classification of animals, phylogenetic relationships (based on classical and molecular phylogenetic tools).

Section 2: Evolution

Origin and history of life on earth, theories of evolution, natural selection, adaptation, speciation.

Section 3: Genetics

Basic Principles of inheritance, the molecular basis of heredity, sex determination and sex-linked characteristics, cytoplasmic inheritance, linkage, recombination and mapping of genes in eukaryotes, population genetics, genetic disorders, roles of model organisms in understanding genetic principles.

Section 4: Biochemistry and Molecular Biology

Nucleic acids, proteins, lipids and carbohydrates; replication, transcription and translation, Krebs cycle, glycolysis, enzyme catalysis, hormones and their actions, roles of vitamins and minerals.

Section 5: Cell Biology

Basic principles of cellular microscopy, the structure of cells, cytoskeletal organization, cellular organelles and their structure and function, cell cycle, cell division, chromosomes and chromatin structure.

Section 6: Gene expression in Eukaryotes

Eukaryotic genome organization and regulation of gene expression, transposable elements.

Section 7: Animal Anatomy and Physiology

Comparative physiology, the respiratory system, Muscular system, circulatory system, digestive system, the nervous system, the excretory system, the endocrine system, the reproductive system, and the skeletal system.

Section 8: Parasitology and Immunology

Nature of parasite, host-parasite relation, protozoan and helminthic parasites, the immune response, cellular and humoral immune response.

Section 9: Development Biology

Gametogenesis, Embryonic development, cellular differentiation, organogenesis, metamorphosis, Model organisms used in developmental biology, the genetic and molecular basis of development, stem cells.

Section 10: Ecology

The ecosystem, Animal distribution, ecological niche and its contribution to ecological diversity, the food chain, population dynamics, species diversity, zoogeography, biogeochemical cycles, conservation biology, and ecotoxicology.

Section 11: Animal Behaviour

Type of behaviours, courtship, mating and territoriality, instinct, learning and memory, social behaviour across the animal taxa, communication, pheromones, and evolution of behaviour in animals.

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GATE 2025 Life Sciences Syllabus - Food Technology

Section 1: Food Chemistry and Nutrition

Carbohydrates: structure and functional properties of mono-, oligo-, & poly saccharides including starch, cellulose, pectic substances and dietary fibre, gelatinization and retrogradation of starch. Proteins: classification and structure of proteins in food, biochemical changes in post-mortem and tenderization of muscles. Lipids: classification and structure of lipids, rancidity, polymerization and polymorphism. Pigments: carotenoids, chlorophylls, anthocyanins, tannins and myoglobin. Food flavours: terpenes, esters, aldehydes, ketones and quinones. Enzymes: specificity, simple and inhibition kinetics, coenzymes, enzymatic and non-enzymatic browning. Nutrition: balanced diet, essential amino acids and essential fatty acids, protein efficiency ratio, water-soluble and fat-soluble vitamins, the role of minerals in nutrition, co-factors, anti-nutrients, nutraceuticals, nutrient deficiency diseases. Chemical and biochemical changes: changes occur in foods during different processing.

Section 2: Food Microbiology

Characteristics of microorganisms: morphology of bacteria, yeast, mould and actinomycetes, spores and vegetative cells, gram-staining. Microbial growth: growth and death kinetics, serial dilution technique. Food spoilage: spoilage microorganisms in different food products including milk, fish, meat, egg, cereals and their products. Toxins from microbes: pathogens and non-pathogens including Staphylococcus, Salmonella, Shebelle, Escherichia, Bacillus, Clostridium, and Aspergillus genera. Fermented foods and beverages: curd, yoghurt, cheese, pickles, soya sauce, sauerkraut, idly, dose, vinegar, alcoholic beverages and sausage.

Section 3: Food Products Technology

Processing principles: thermal processing, chilling, freezing, dehydration, addition of preservatives and food additives, irradiation, fermentation, hurdle technology, intermediate moisture foods. Food packaging and storage: packaging materials, aseptic packaging, controlled and modified atmosphere storage. Cereal processing and products: milling of rice, wheat, and maize, parboiling of paddy, bread, biscuits, extruded products and ready-to-eat breakfast cereals. Oil processing: expelling, solvent extraction, refining and hydrogenation. Fruits and vegetable processing: extraction, clarification, concentration and packaging of fruit juice, jam, jelly, marmalade, squash, candies, tomato sauce, ketchup, and puree, potato chips, pickles. Plantation crops processing and products: tea, coffee, cocoa, spice, extraction of essential oils and oleoresins from spices. Milk and milk products processing: pasteurization and sterilization, cream, butter, ghee, ice- cream, cheese and milk powder. Processing of animal products: drying, canning, and freezing of fish and meat; production of egg powder. Waste utilization: pectin from fruit wastes, uses of by-products from rice milling. Food standards and quality maintenance: FPO, PFA, A-Mark, ISI, HACCP, food plant sanitation and cleaning in place (CIP).

Section 4: Food Engineering

Mass and energy balance: Momentum transfer: Flow rate and pressure drop relationships for Newtonian fluids flowing through a pipe, Reynolds number. Heat transfer: heat transfer by conduction, convection, radiation, heat exchangers. Mass transfer: molecular diffusion and Flick's law, conduction and convective mass transfer, permeability through single and multilayer films. Mechanical operations: size reduction of solids, high-pressure homogenization, filtration,

centrifugation, settling, sieving, mixing & agitation of liquid. Thermal operations: thermal sterilization, evaporation of liquid foods, hot air drying of solids, spray and freeze-drying, freezing and crystallization. Mass transfer operations: psychometric, humidification and dehumidification operations.

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